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Five-micron tissue sections were made by rotatory microtome and mounted on glass slides for hematoxylin and eosin (H&E) staining and general light microscopic histological examination of tissue samples as well as Massons Goldners trichrome staining utilized for differentiation of mineralized and non-mineralized areas in bone matrix

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The results were analysed using the threshold cycle (Ct) value to obtain the relative expression of the target gene and the internal reference gene (16S rRNA)

Brain Res Brain Res Rev (2005) 48:45776